SPATA19 Polyklonaler Antikörper

SPATA19 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16656-1-AP

Synonyme

SPAS1, SPATA19, Spergen 1, spergen1, spermatogenesis associated 19

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Galerie der Validierungsdaten

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  WB analysis of human testis using 16656-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16656-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16656-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16656-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16656-1-AP (SPATA19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16656-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16656-1-AP (SPATA19 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse testis using 16656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 16656-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPATA19 antibody (16656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

16656-1-AP bindet in WB, IHC, IF, ELISA SPATA19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPATA19 fusion protein Ag10108
• Vollständiger Name: spermatogenesis associated 19
• Berechnetes Molekulargewicht: 167 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC058039
• Gene symbol: SPATA19
• Gene ID (NCBI): 219938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
mouse	WB,IF	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang