SPANXA2/SPANXA1 Polyklonaler Antikörper

SPANXA2/SPANXA1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19527-1-AP

Synonyme

Cancer/testis antigen 11.1, CT11.1, SPAN X, SPANX, SPANX A, SPANX family member A, SPANX family, member A2, SPANXA, SPANXA1, SPANXA2

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Galerie der Validierungsdaten

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  WB analysis of DU 145 using 19527-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19527-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19527-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19527-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

• 

  IHC staining of human skin cancer using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

• 

  IHC staining of human testis using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

• 

  IHC staining of human skin cancer using 19527-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50.

• 

  IF Staining of HEK-293 using 19527-1-AP

  Immunofluorescent analysis of HEK-293 cells using 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19527-1-AP bindet in WB, IHC, IF,ELISA SPANXA2/SPANXA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SPANX family, member A2
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: NM_145662
• Gene symbol: SPANXA2
• Gene ID (NCBI): 728712
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPANXA1, also named as SPANXA and SPANXA2, is a member of the SPANX family of cancer/testis-associated genes. It encodes differentially expressed testis-specific proteins that localize to various subcellular compartments. On Western blots of human testis proteins, SPANX antibodies reacts with the bands migrating between 15-30 kDa. The SPANX protein may be a useful target in cancer immunotherapy.