SPAG5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SPAG5 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 14726-1-AP

Synonyme

Astrin, DEEPEST, hMAP126, MAP126, SPAG5, sperm associated antigen 5

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Galerie der Validierungsdaten

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  WB analysis using 14726-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14726-1-AP (SPAG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14726-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 14726-1-AP (SPAG5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14726-1-AP

  IP Result of anti-SPAG5 (IP:14726-1-AP, 5ug; Detection:14726-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human testis using 14726-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14726-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse testis using 14726-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14726-1-AP (SPAG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14726-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (14726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, K-562-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 2 publications below

Produktinformation

14726-1-AP bindet in WB, IP, IHC, IF, ELISA SPAG5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG5 fusion protein Ag6485
• Vollständiger Name: sperm associated antigen 5
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: 135 kDa, 150 kDa
• GenBank-Zugangsnummer: BC000322
• Gene symbol: SPAG5
• Gene ID (NCBI): 10615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

Publikationen

Species	Application	Title
mouse,human	WB,IF	Nat Commun; CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.; Authors - Diego R Revinski
human	WB, IP, IF	Elife; Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.; Authors - Andrew Kodani ; KD Validated
human	WB	Elife; Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation.; Authors - Duccio Conti
human	WB,IP	Elife; CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression.; Authors - Désirée Schatton
mouse	IHC,IF	Elife; DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction.; Authors - Eva On-Chai Lau
human	WB,IHC	J Nanobiotechnology; Fe-doped chrysotile nanotubes containing siRNAs to silence SPAG5 to treat bladder cancer.; Authors - Jianye Liu