SPACA3 Polyklonaler Antikörper

SPACA3 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21137-1-AP

Synonyme

1700025M08Rik, ALLP17, Cancer/testis antigen 54, CT54, LYC3, Lysozyme like protein 3, LYZL3, SLLP1, SPACA3, sperm acrosome associated 3, Sperm lysozyme like protein 1, SPRASA

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 21137-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21137-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21137-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 21137-1-AP

  Immunofluorescent analysis of HEK-293 cells using 21137-1-AP (SPACA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21137-1-AP bindet in WB, IF,ELISA SPACA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPACA3 fusion protein Ag15589
• Vollständiger Name: sperm acrosome associated 3
• Berechnetes Molekulargewicht: 215 aa, 23 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa, 46-50 kDa
• GenBank-Zugangsnummer: BC029867
• Gene symbol: SPACA3
• Gene ID (NCBI): 124912
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPACA3, also named as LYC3, LYZL3, SLLP1, SPRASA, CT54 and ALLP-17, belongs to the glycosyl hydrolase 22 family. It is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. SPACA3 has a Dimer form(PMID:15982649 ).