SP3 Polyklonaler Antikörper

SP3 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26584-1-AP

Synonyme

SP3, Sp3 transcription factor, SPR 2, Transcription factor Sp3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen, COLO 320-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 5 publications below
IHCSee 1 publications below

Produktinformation

26584-1-AP bindet in WB, IHC, ELISA SP3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SP3 fusion protein Ag25082
Vollständiger Name Sp3 transcription factor
Berechnetes Molekulargewicht 781 aa, 82 kDa
Beobachtetes Molekulargewicht 70 kDa, 115 kDa
GenBank-ZugangsnummerBC126414
Gene symbol SP3
Gene ID (NCBI) 6670
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SP3, also named as transcription factor Sp3, is a 781 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. SP3 localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies. SP3 is acetylated by histone acetyltransferase p300, deacetylated by HDACs and sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. There exits tow modified isoform and molecular weight of those are 70 kda and 115 kda.

Protokolle

Produktspezifische Protokolle
WB protocol for SP3 antibody 26584-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Dig Dis Sci

Speckle-Type POZ Protein Down-Regulates Matrix Metalloproteinase 2 Expression via Sp1/PI3K/Akt Signaling Pathway in Colorectal Cancer.

Authors - Shouhua Zhang
humanWB

Biochem Biophys Res Commun

Transcriptional activation of CSTB gene expression by transcription factor Sp3

Authors - Weiyi Zhu
humanWB,IHC

Mol Cell Probes

LINC00115 regulates lung adenocarcinoma progression via sponging miR-154-3p to modulate Sp3 expression

Authors - Kexin Sun
mouseWB

Cell Rep

LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis

Authors - Longze Sha
human,mouseWB

Int Immunopharmacol

Menstrual blood-derived stem cells exosomal miR-let-7 to ameliorate pulmonary fibrosis through inhibiting ferroptosis by Sp3/HDAC2/Nrf2 signaling pathway

Authors - Lifang Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anh (Verified Customer) (07-15-2021)

clear bands, have some unspecific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse heart
SP3 Antibody Western Blot validation (1:500 dilution) in mouse heart (Cat no:26584-1-AP)