SP3 Polyklonaler Antikörper
SP3 Polyklonal Antikörper für WB,ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 26584-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | PC-3-Zellen, COLO 320-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 5 publications below |
IHC | See 1 publications below |
Produktinformation
26584-1-AP bindet in WB, IHC, ELISA SP3 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | SP3 fusion protein Ag25082 |
Vollständiger Name | Sp3 transcription factor |
Berechnetes Molekulargewicht | 781 aa, 82 kDa |
Beobachtetes Molekulargewicht | 70 kDa, 115 kDa |
GenBank-Zugangsnummer | BC126414 |
Gene symbol | SP3 |
Gene ID (NCBI) | 6670 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
SP3, also named as transcription factor Sp3, is a 781 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. SP3 localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies. SP3 is acetylated by histone acetyltransferase p300, deacetylated by HDACs and sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. There exits tow modified isoform and molecular weight of those are 70 kda and 115 kda.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for SP3 antibody 26584-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Dig Dis Sci Speckle-Type POZ Protein Down-Regulates Matrix Metalloproteinase 2 Expression via Sp1/PI3K/Akt Signaling Pathway in Colorectal Cancer. | ||
Biochem Biophys Res Commun Transcriptional activation of CSTB gene expression by transcription factor Sp3 | ||
Mol Cell Probes LINC00115 regulates lung adenocarcinoma progression via sponging miR-154-3p to modulate Sp3 expression | ||
Cell Rep LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis | ||
Int Immunopharmacol Menstrual blood-derived stem cells exosomal miR-let-7 to ameliorate pulmonary fibrosis through inhibiting ferroptosis by Sp3/HDAC2/Nrf2 signaling pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anh (Verified Customer) (07-15-2021) | clear bands, have some unspecific bands
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