SP1 Monoklonaler Antikörper

SP1 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4G3H3

Kat-Nr. : 66508-1-Ig

Synonyme

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

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Galerie der Validierungsdaten

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  WB analysis using 66508-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66508-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66508-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66508-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66508-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66508-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66508-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HEK-293 using 66508-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HEK-293 using 66508-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SP1 (66508-1-Ig, Clone:4G3H3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe, HEK-293-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:175-1:700
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

66508-1-Ig bindet in WB, IHC, IF, FC, ELISA SP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SP1 fusion protein Ag16720
• Vollständiger Name: Sp1 transcription factor
• Berechnetes Molekulargewicht: 785 aa, 81 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa, 60-65 kDa
• GenBank-Zugangsnummer: BC062539
• Gene symbol: SP1
• Gene ID (NCBI): 6667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

Publikationen

Species	Application	Title
rat	WB	Br J Pharmacol; Sarco/endoplasmic reticulum Ca 2+ -ATPase 2b mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain; Authors - Shaoheng Li
mouse	IHC	Evid Based Complement Alternat Med; Guifu Dihuang Pills Ameliorated Mucus Hypersecretion by Suppressing Muc5ac Expression and Inactivating the ERK-SP1 Pathway in Lipopolysaccharide/Cigarette Smoke-Induced Mice; Authors - Huanhuan Zhang