SOX2 Monoklonaler Antikörper

SOX2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4B9B10

Kat-Nr. : CL594-66411

Synonyme

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

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Galerie der Validierungsdaten

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  IF Staining of mouse brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66411 (SOX2 antibody) at dilution of 1:50.

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  IF Staining of rat brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:400.

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  IF Staining of mouse brain using CL594-66411

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX2 antibody (CL594-66411, Clone: 4B9B10 ) at dilution of 1:200. DAPI (blue).

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  FC experiment of U-251 using CL594-66411

  1X10^6 U-251 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SOX2 (CL594-66411, Clone:4B9B10) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC: U-251-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66411 bindet in IF, FC (Intra) SOX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SOX2 fusion protein Ag13635
• Vollständiger Name: SRY (sex determining region Y)-box 2
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC013923
• Gene symbol: SOX2
• Gene ID (NCBI): 6657
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011