SOX2 Monoklonaler Antikörper

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• KD/KO Validated

SOX2 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

34

CloneNo.

4B9B10

Kat-Nr. : 66411-1-Ig

Synonyme

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

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Galerie der Validierungsdaten

• 

  WB analysis using 66411-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66411-1-Ig

  WB result of SOX2 antibody (66411-1-Ig, 1:4000) with si-Control and si-SOX2 transfected HEK293 cells..

• 

  WB analysis of U-251 using 66411-1-Ig

  WB result of SOX2 antibody (66411-1-Ig; 1:3000; room temperature for 1.5 hours) with negative control and SOX2 knockout U-251 cells.

• 

  WB analysis of MCF-7 using 66411-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66411-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66411-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66411-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66411-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse embryo using 66411-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 66411-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 66411-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66411-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66411-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66411-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66411-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66411-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66411-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66411-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of U-251 using 66411-1-Ig

  1X10^6 U-251 cells were intracellularly stained with 0.5 ug Anti-Human SOX2 (66411-1-Ig, Clone:4B9B10) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, C6-Zellen, fetales humanes Hirngewebe, Hausschwein-Hirngewebe, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Mausembryogewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe
• Erfolgreiche Detektion in FC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 26 publications below
• IHC: See 1 publications below
• IF: See 11 publications below

Produktinformation

66411-1-Ig bindet in WB, IHC, IF, FC, ELISA SOX2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SOX2 fusion protein Ag13635
• Vollständiger Name: SRY (sex determining region Y)-box 2
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC013923
• Gene symbol: SOX2
• Gene ID (NCBI): 6657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

SRY (sex determining region Y)-box 2 is a transcription factor involved in neural stem cell maintenance and has been used in somatic cell reprogramming.

 What is the molecular weight of Sox2?

34 kDa. Sox2 is an intronless gene encoding xx amino acids including HMG (High-mobility group) box domains that can bind to DNA.

 What is the tissue specificity of Sox2?

In embryonic stem cells, Sox2 is highly expressed and is essential in self-renewal. In the developing embryo, Sox2 is found in the neural tube and then in the developing eye and central nervous system (CNS). This expression is highly conserved across organisms (PMID: 25126380; PMID: 8625802).

In adult tissues Sox2 is expressed in neural progenitor cells, which as multipotent cells can both self-renew and differentiate into neurons, astroglia, and oligodendroglia. It maintains their stem cell phenotype and is a useful marker of early neural stem cells (PMID: 16139372).

 What is the role of Sox2 in pluripotency?

Sox2 acts as part of the LIF (Leukemia inhibitory factor) pathway, activated via JAK-STAT signaling to form complexes with other transcription factors such as Oct4 that bind to DNA and work to control pluripotency. Sox2 was described as one of the four original factors that are key to the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (PMID: 16904174).

 What is the role of Sox2 in cancer?

The ability to control pluripotency means dysregulation of Sox2 can lead to uncontrolled proliferation. In many different cancers, Sox2 has been associated with the metastasis and growth of tumors, mostly due to overexpression (PMID: 28388544). The increased levels of Sox2 RNA and protein in tumor tissue highlight it as a potential therapeutic target (PMID: 25114775).

Publikationen

Species	Application	Title
rat	IF	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
mouse	IF	Bone Res; Type II collagen-positive progenitors are important stem cells in controlling skeletal development and vascular formation.; Authors - Xinhua Li
human	WB	Nanoscale; An assembly-inducing PDC enabling the efficient nuclear delivery of nucleic acid for cancer stem-like cell suppression; Authors - Dongyuan Wang
human	WB	Oncogene; MSC-regulated lncRNA MACC1-AS1 promotes stemness and chemoresistance through fatty acid oxidation in gastric cancer.; Authors - Wanming He
human	WB	Int J Mol Sci; Addressing Doxorubicin Resistance in Bone Sarcomas Using Novel Drug-Resistant Models.; Authors - Borja Gallego
mouse	IF,WB	iScience; Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency; Authors - Qian Ban