SOX2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SOX2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Rezensionen (3)

Kat-Nr. : 20118-1-AP

Synonyme

ANOP3, MCOPS3, SOX2, Transcription factor SOX 2

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20118-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20118-1-AP (SOX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20118-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20118-1-AP (SOX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20118-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20118-1-AP (SOX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 20118-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 20118-1-AP (SOX2 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 20118-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 20118-1-AP (SOX2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20118-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20118-1-AP (SOX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse embryo using 20118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20118-1-AP (SOX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 20118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20118-1-AP (SOX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20118-1-AP (SOX2 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20118-1-AP (SOX2 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 20118-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20118-1-AP (SOX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 20118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20118-1-AP (SOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20118-1-AP (SOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 20118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 20118-1-AP (SOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 20118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 20118-1-AP (SOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse embryo using 20118-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 20118-1-AP (SOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NCCIT using 20118-1-AP

  1X10^6 NCCIT cells were intracellularly stained with 0.5 ug Anti-Human SOX2 (20118-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausembryogewebe, Rattenhirngewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausembryogewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, Mausembryogewebe
• Erfolgreiche Detektion in FC: NCCIT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

20118-1-AP bindet in WB, IHC, IF, FC, ELISA SOX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOX2 fusion protein Ag13635
• Vollständiger Name: SRY (sex determining region Y)-box 2
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC013923
• Gene symbol: SOX2
• Gene ID (NCBI): 6657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

SRY (sex determining region Y)-box 2 is a transcription factor involved in neural stem cell maintenance and has been used in somatic cell reprogramming.

 What is the molecular weight of Sox2?

34 kDa. Sox2 is an intronless gene encoding xx amino acids including HMG (High-mobility group) box domains that can bind to DNA.

 What is the tissue specificity of Sox2?

In embryonic stem cells, Sox2 is highly expressed and is essential in self-renewal. In the developing embryo, Sox2 is found in the neural tube and then in the developing eye and central nervous system (CNS). This expression is highly conserved across organisms (PMID: 25126380; PMID: 8625802).

In adult tissues Sox2 is expressed in neural progenitor cells, which as multipotent cells can both self-renew and differentiate into neurons, astroglia, and oligodendroglia. It maintains their stem cell phenotype and is a useful marker of early neural stem cells (PMID: 16139372).

 What is the role of Sox2 in pluripotency?

Sox2 acts as part of the LIF (Leukemia inhibitory factor) pathway, activated via JAK-STAT signaling to form complexes with other transcription factors such as Oct4 that bind to DNA and work to control pluripotency. Sox2 was described as one of the four original factors that are key to the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (PMID: 16904174).

 What is the role of Sox2 in cancer?

The ability to control pluripotency means dysregulation of Sox2 can lead to uncontrolled proliferation. In many different cancers, Sox2 has been associated with the metastasis and growth of tumors, mostly due to overexpression (PMID: 28388544). The increased levels of Sox2 RNA and protein in tumor tissue highlight it as a potential therapeutic target (PMID: 25114775).

Publikationen

Species	Application	Title
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
human	IF	Int J Mol Sci; Patients' Stem Cells Differentiation in a 3D Environment as a Promising Experimental Tool for the Study of Amyotrophic Lateral Sclerosis.; Authors - Eveljn Scarian
human	WB	Cell Death Dis; Linc-DYNC2H1-4 promotes EMT and CSC phenotypes by acting as a sponge of miR-145 in pancreatic cancer cells.; Authors - Yuran Gao
	WB	Sci Rep; Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells.; Authors - Yu-Che Cheng
human	WB	Cell Cycle; LncRNA SOX2OT affects cervical cancer cell growth, migration and invasion by regulating SOX2.; Authors - Xiaohan Chang
human	WB	J Cell Mol Med; Epigenetic reprogramming of human lung cancer cells with the extract of bovine parthenogenetic oocytes.; Authors - Zhenfei Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jacob (Verified Customer) (05-08-2023)

good signal in D20 old neuronal organoids

• Applications: Immunofluorescence
• Cell Tissue Type: neuronal organoid

FH

Lisa (Verified Customer) (11-07-2022)

Proteintech is always a guarantee of good antibodies! The antibodies works very well for IF, not yet tested for WB.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: hiPSCs-derived neuronal progenitor cells

FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph=6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Zebrafish retina 5dpf

SOX2 Antibody Immunohistochemistry, validation (1:200 dilution) in Zebrafish retina 5dpf (Cat no:20118-1-AP)