SOS1 Polyklonaler Antikörper

SOS1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 55041-1-AP

Synonyme

GF1, GGF1, GINGF, NS4, Son of sevenless homolog 1, SOS 1, SOS1

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Galerie der Validierungsdaten

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  WB analysis using 55041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55041-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55041-1-AP

  IP Result of anti-SOS1 (IP:55041-1-AP, 4ug; Detection:55041-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse colon using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55041-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOS1 antibody (55041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushirngewebe, SKOV-3-Zellen, THP-1-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

55041-1-AP bindet in WB, IP, IHC, IF, ELISA SOS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: son of sevenless homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 152 kDa
• Beobachtetes Molekulargewicht: 152 kDa
• GenBank-Zugangsnummer: NM_005633
• Gene symbol: SOS1
• Gene ID (NCBI): 6654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.

Publikationen

Species	Application	Title
human	WB	Cells; Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.; Authors - Damian Kołat
human	WB	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang