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SOD2 Monoklonaler Antikörper

SOD2 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

3A6C2

Kat-Nr. : CL555-66474

Synonyme

IPO B, Mn SOD, MNSOD, SOD2, SODM

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated SOD2 Monoclonal antibody (CL555-66474)

    FC experiment of HeLa using CL555-66474

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human SOD2 (CL555-66474, Clone:3A6C2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66474 bindet in FC (Intra) SOD2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen SOD2 fusion protein Ag21388
Vollständiger Name superoxide dismutase 2, mitochondrial
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protokolle

Produktspezifische Protokolle
FC protocol for CL555 SOD2 antibody CL555-66474Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Anja (Verified Customer) (07-25-2022)

The antibody worked well for Flow Cytometry at a 1:100 dilution for isolated mouse cells. I would buy it again.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Thymic Epithelial Cells