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SOD2 Monoklonaler Antikörper

SOD2 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3A6C2

Kat-Nr. : 66474-1-Ig

Synonyme

IPO B, Mn SOD, MNSOD, SOD2, SODM



Geprüfte Anwendungen

Erfolgreiche Detektion in WBU2OS-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe, RAW 264.7-Zellen, ROS1728-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Pankreaskarzinomgewebe, Mauslebergewebe, Rattenlebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHUVEC-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:2500-1:10000
Immunfluoreszenz (IF)IF : 1:200-1:800
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66474-1-Ig bindet in WB, IHC, IF, FC, CoIP, ELISA SOD2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte, Ziege
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen SOD2 fusion protein Ag21388
Vollständiger Name superoxide dismutase 2, mitochondrial
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protokolle

Produktspezifische Protokolle
WB protocol for SOD2 antibody 66474-1-IgProtokoll herunterladen
IHC protocol for SOD2 antibody 66474-1-IgProtokoll herunterladen
IF protocol for SOD2 antibody 66474-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB,IF

J Control Release

A cerium oxide loaded glycol chitosan nano-system for the treatment of dry eye disease.

Authors - Fan Yu
mouseWB

Stem Cell Res Ther

Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathway

Authors - Xiaotong Huang
humanWB

Oxid Med Cell Longev

HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation.

Authors - Shangting Han
mouseWB

Mol Ther Nucleic Acids

Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis

Authors - Ni Zeng
pigWB

J Anim Sci Biotechnol

Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota.

Authors - Yanan Chen
humanWB

J Anim Sci Biotechnol

Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets.

Authors - Hao Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (07-30-2020)

This antibody works very well at 1:2000 dilution with O/N incubation.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Fallopian tube epithelial cells
SOD2 Antibody Western Blot, validation (1:2000 dilution) in Fallopian tube epithelial cells (Cat no:66474-1-Ig)