SNRPA1 Polyklonaler Antikörper

SNRPA1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17368-1-AP

Synonyme

SNRPA1, U2 snRNP A

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17368-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17368-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17368-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17368-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17368-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17368-1-AP (SNRPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17368-1-AP

  IP Result of anti-SNRPA1 (IP:17368-1-AP, 4ug; Detection:17368-1-AP 1:500) with HepG2 cells lysate 1000ug.

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  IHC staining of human lung using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17368-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17368-1-AP (SNRPA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17368-1-AP

  Immunofluorescent analysis of HepG2 cells, using SNRPA1 antibody 17368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

17368-1-AP bindet in WB, IP, IHC, IF, ELISA SNRPA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNRPA1 fusion protein Ag10790
• Vollständiger Name: small nuclear ribonucleoprotein polypeptide A'
• Berechnetes Molekulargewicht: 255 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC022816
• Gene symbol: SNRPA1
• Gene ID (NCBI): 6627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Inactivation of the tumor suppressor p53 by long noncoding RNA RMRP.; Authors - Yajie Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Cell Rep; SF3B1 Association with Chromatin Determines Splicing Outcomes.; Authors - Nir Kfir
human	WB	STAR Protoc; Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.; Authors - Li Wang
human	WB,IHC	Clin Respir J; High SNRPA1 expression leads to poor prognosis in patients with lung adenocarcinoma; Authors - Juan Juan Yang