SNF2L Polyklonaler Antikörper

SNF2L Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 29461-1-AP

Synonyme

ATP dependent helicase SMARCA1, DKFZp686D1623, FLJ41547, ISWI, NURF140, SMARCA1, SNF2L, SNF2L1, SNF2LB, SWI, SWI2

---

• 

  WB analysis using 29461-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 29461-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 29461-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat lung using 29461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 29461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Rattenlungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29461-1-AP bindet in WB, IHC, ELISA SNF2L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNF2L fusion protein Ag30657
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 120-130 KDa
• GenBank-Zugangsnummer: NM_003069
• Gene symbol: SMARCA1/SNF2L
• Gene ID (NCBI): 6594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Death Differ; BAP18 facilitates CTCF-mediated chromatin accessible to regulate enhancer activity in breast cancer; Authors - Ge Sun