SMYD3 Monoklonaler Antikörper

SMYD3 Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5C10

Kat-Nr. : CL488-66330

Synonyme

bA74P14.1, FLJ21080, SMYD3, ZMYND1, ZNFN3A1

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Galerie der Validierungsdaten

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  WB analysis using CL488-66330

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66330 (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66330 bindet in WB SMYD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMYD3 fusion protein Ag2624
• Vollständiger Name: SET and MYND domain containing 3
• Berechnetes Molekulargewicht: 428aa,49 kDa; 369aa,42 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC031010
• Gene symbol: SMYD3
• Gene ID (NCBI): 64754
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) .