SMYD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMYD2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 21290-1-AP

Synonyme

HSKM B, KMT3C, Lysine N methyltransferase 3C, SMYD2, ZMYND14

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Galerie der Validierungsdaten

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  WB analysis using 21290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat heart using 21290-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21290-1-AP (SMYD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 21290-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21290-1-AP (SMYD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMYD2 antibody (21290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12 cell, C2C12-Zellen, HEK-293-Zellen, Mausherzgewebe, Rattenherzgewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Produktinformation

21290-1-AP bindet in WB, IHC, IF, ELISA SMYD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMYD2 fusion protein Ag15823
• Vollständiger Name: SET and MYND domain containing 2
• Berechnetes Molekulargewicht: 433 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC098305
• Gene symbol: SMYD2
• Gene ID (NCBI): 56950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMYD2, also known as SET and MYND domain containing 2, encodes an enzyme called N-lysine methyltransferase SMYD2, which plays a crucial role in cellular processes, including histone methylation, non-histone protein methylation, cell proliferation and differentiation, DNA repair (PMID: 18065756, PMID: 36191822). Aberrant SMYD2 expression has been linked to various cancers, including colon cancer, lung cancer, and cervical cancer. Smyd2 is strongly expressed in muscle cells with highest expression in the neonatal heart (PMID: 25125178).

Publikationen

Species	Application	Title
mouse	IHC	Cell Death Dis; SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth.; Authors - Yu-Qiang Yu
human	IHC	Theranostics; Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma.; Authors - Libin Yan ; KD Validated
human	WB	Stem Cells; SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells through Mediating the Transcriptional Activation of Key Mesendodermal Genes.; Authors - Hua-Jun Bai ; KO Validated
human	WB,IHC	Am J Cancer Res; SMYD2 suppresses APC2 expression to activate the Wnt/β-catenin pathway and promotes epithelial-mesenchymal transition in colorectal cancer.; Authors - Fanchao Meng ; KD Validated
mouse	WB,IHC,IF	J Pharmacol Sci; Pharmacological inhibition of SMYD2 protects against cisplatin-induced renal fibrosis and inflammation; Authors - Min Chen
human	WB	Exp Cell Res; SMYD2 suppresses p53 activity to promote glucose metabolism in cervical cancer.; Authors - Ying Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

This antibody worked well in the differentiated human cardiomyocytes.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human cardiomyocytes