SMYD1-Specific Polyklonaler Antikörper

SMYD1-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (2)

Kat-Nr. : 16151-1-AP

Synonyme

BOP, SMYD1, SMYD1-Specific, ZMYND18, ZMYND22

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 16151-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16151-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16151-1-AP

  IP Result of anti-SMYD1-Specific (IP:16151-1-AP, 4ug; Detection:16151-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human testis using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16151-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16151-1-AP (SMYD1-Specific antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

16151-1-AP bindet in WB, IP, IHC, IF, ELISA SMYD1-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SET and MYND domain containing 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: NM_198274
• Gene symbol: SMYD1
• Gene ID (NCBI): 150572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Toxicol Lett; Down-regulated Smyd1 participated in the inhibition of myoblast differentiation induced by cigarette smoke extract; Authors - Fang Li ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

SMYD1 antibody worked very well in human differentiated cardiomyocyte.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Human cardiomyocytes

FH

Eric (Verified Customer) (10-15-2018)

Very low detection of SMYD1: 45 ug protein, overnight incubation with primary antibody at 4 oC, 2h incubation with secondary at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: C2C12/mouse heart