SMU1 Polyklonaler Antikörper

SMU1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15511-1-AP

Synonyme

BWD, fSAP57, RP11 54K16.3, SMU 1, SMU1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 15511-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15511-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15511-1-AP (SMU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15511-1-AP (SMU1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15511-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15511-1-AP (SMU1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15511-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMU1 antibody 15511-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15511-1-AP bindet in WB, IHC, IF,ELISA SMU1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMU1 fusion protein Ag7688
• Vollständiger Name: smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC002876
• Gene symbol: SMU1
• Gene ID (NCBI): 55234
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sum1, belongs to the WD repeat SMU1 family. Human SMU1 is a component of the spliceosome fSAP57(PMID: 15254247). It is possible that the mutation in Smu1 affects spliceosome function, resulting in generation of improper splice variants, which could explain the phenotypes of tsTM18 cells. Some results suggest that Smu1 participates in at least two steps of splicing: splice-site choice and control of accuracy(PMID: 17105761). This antibody is a rabbit polyclonal antibody raised against a region of human REDD1 antigen.