SMPD1,ASM Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMPD1,ASM Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 14609-1-AP

Synonyme

ASM, SMPD1, SMPD1,ASM

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Galerie der Validierungsdaten

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  WB analysis using 14609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14609-1-AP (SMPD1,ASM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14609-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14609-1-AP (SMPD1,ASM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Produktinformation

14609-1-AP bindet in WB, IHC, IF, ELISA SMPD1,ASM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMPD1,ASM fusion protein Ag6180
• Vollständiger Name: sphingomyelin phosphodiesterase 1, acid lysosomal
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 70-75 kDa
• GenBank-Zugangsnummer: BC041164
• Gene symbol: SMPD1
• Gene ID (NCBI): 6609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.

Publikationen

Species	Application	Title
mouse		Cell Host Microbe; The β2Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.; Authors - Alexander Gluschko ; KO Validated
human	WB,IF	Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang
mouse	IF	Acta Neuropathol Commun; Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse; Authors - Simone M Crivelli
mouse	IF	Cells; Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease; Authors - Ahmed Elsherbini
human	IHC	J Mol Med (Berl); Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma; Authors - Gregory C Wilson

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alice (Verified Customer) (05-11-2021)

Good Ab should show good result in your website to convince people to use it.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Pancreas

SMPD1,ASM Antibody Immunohistochemistry validation (1:200 dilution) in Mouse Pancreas (Cat no:14609-1-AP)