SMOX Polyklonaler Antikörper

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• KD/KO Validated

SMOX Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 15052-1-AP

Synonyme

C20orf16, PAO, PAO 1, PAOh1, Polyamine oxidase 1, SMO, SMOX, spermine oxidase

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Galerie der Validierungsdaten

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  WB analysis using 15052-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 15052-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15052-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15052-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, MCF-7-Zellen, PC-13-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Gliomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

15052-1-AP bindet in WB, IHC, IF, ELISA SMOX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMOX fusion protein Ag7035
• Vollständiger Name: spermine oxidase
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 56-65 kDa
• GenBank-Zugangsnummer: BC000669
• Gene symbol: SMOX
• Gene ID (NCBI): 54498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma.; Authors - Anna Bianchi-Smiraglia
mouse	WB	J Clin Invest; Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.; Authors - Leslie A Sandusky-Beltran
mouse	WB	EBioMedicine; Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.; Authors - Guangtian Wang
human	WB	J Invest Dermatol; The Polyamine Putrescine Promotes Human Epidermal Melanogenesis.; Authors - Aishwarya Sridharan
		Free Radic Biol Med; Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: Evaluation of a new transgenic mouse model.; Authors - Roberta Ceci
human	WB,IHC	Biomedicines; Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients.; Authors - Sooyoun Kim