SMC5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMC5 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 14178-1-AP

Synonyme

hSMC5, KIAA0594, SMC 5, SMC protein 5, SMC5, SMC5L1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 14178-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14178-1-AP (SMC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

14178-1-AP bindet in WB, IF, ELISA SMC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMC5 fusion protein Ag5385
• Vollständiger Name: structural maintenance of chromosomes 5
• Berechnetes Molekulargewicht: 1101 aa, 129 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC038225
• Gene symbol: SMC5
• Gene ID (NCBI): 23137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	mSystems; Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes; Authors - Joseph M. Dybas ; KD Validated
human	IF	J Virol; PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.; Authors - Wei Xu
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (11-30-2022)

The detection of SMC5 by WB is fair with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HepG2 cell

SMC5 Antibody Western Blot validation (1/1000 dilution) in HepG2 cell (Cat no:14178-1-AP)