SMARCD1 Monoklonaler Antikörper

SMARCD1 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

2F2G6

Kat-Nr. : 66116-1-Ig

Synonyme

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 66116-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66116-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66116-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, humanes Hirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66116-1-Ig bindet in WB,ELISA SMARCD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMARCD1 fusion protein Ag19100
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC009368
• Gene symbol: SMARCD1
• Gene ID (NCBI): 6602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1), also known as BAF60a (BRG1-associated factor 60A),Rsc6p or CRACD1, is a 476 amino acid protein that localizes to the nucleus and contains one SWIB domain. Expressed ubiquitously with notable expression in liver, brain, muscle, lung, kidney, pancreas and placenta, SMARCD1 functions as a component of the BAF complex and, in conjunction with a variety of other proteins, plays an essential role in chromatin remodeling. In addition, SMARCD1 influences vitamin D-mediated transcriptional activity and is thought to provide a link between the vitamin D receptor (VDR) and SWI/SNF chromatin remodeling complexes. Multiple isoforms of BAF60a exist due to alternative splicing events.