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  • Featured Product
  • KD/KO Validated

SMARCA2-Specific Polyklonaler Antikörper

SMARCA2-Specific Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-26613

Synonyme

ATP dependent helicase SMARCA2, BAF190, BAF190B, BRG1 associated factor 190B, BRM, FLJ36757, hBRM, hSNF2a, Protein brahma homolog, SMARCA2, SNF2, SNF2 alpha, SNF2A, SNF2L2, SNF2LA, Sth1p, SWI2

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SMARCA2-Specific Pol (CL488-26613)

    FC experiment of HeLa using CL488-26613

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SMARCA2-Specific (CL488-26613) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-26613 bindet in FC (Intra) SMARCA2-Specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SMARCA2-Specific fusion protein Ag25157
Vollständiger Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Berechnetes Molekulargewicht 181 kDa
Beobachtetes Molekulargewicht 180-190 kDa
GenBank-ZugangsnummerNM_003070
Gene symbol SMARCA2
Gene ID (NCBI) 6595
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.