SLC7A5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC7A5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 28670-1-AP

Synonyme

4F2 LC, 4F2 light chain, 4F2LC, CD98 light chain, CD98LC, D16S469E, E16, hLAT1, Integral membrane protein E16, LAT1, MPE16, SLC7A5

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Galerie der Validierungsdaten

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  WB analysis using 28670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28670-1-AP (SLC7A5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 28670-1-AP

  WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

• 

  WB analysis of MCF-7 using 28670-1-AP

  WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

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  IHC staining of human lung cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, MKN-45-Zellen, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

28670-1-AP bindet in WB, IHC, ELISA SLC7A5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC7A5 fusion protein Ag29164
• Vollständiger Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Berechnetes Molekulargewicht: 507 aa, 55 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC039692
• Gene symbol: SLC7A5
• Gene ID (NCBI): 8140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Inherit Metab Dis; Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission; Authors - Sofía Illescas
human	WB	J Exp Clin Cancer Res; CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex; Authors - Qiang Ma
human	IHC,WB	Front Oncol; ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation; Authors - Daqi Jia
human	WB	Sci Adv; Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells; Authors - Dennis J Hsu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xie (Verified Customer) (07-07-2023)

The expression of Slc7a5 is good for my samples

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: brain

SLC7A5 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:28670-1-AP)