CAT-1 Polyklonaler Antikörper

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• KD/KO Validated

CAT-1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 14195-1-AP

Synonyme

Amino acid transporter cationic 1, ATRC1, CAT 1, CAT1, CAT-1, HCAT1, REC1L, SLC7A1

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Galerie der Validierungsdaten

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  WB analysis of rat brain/liver tissue using 14195-1-AP

  WB result of 14195-1-AP from Dilwar in Rat tissue. Rat brain (a), heart (b) liver (c) mitochondria and liver homogenate (d), (40 ug with 10% PAGE) probed with 1:1000 dilution.

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  WB analysis using 14195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14195-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14195-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14195-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Jurkat-Zellen, Mausherzgewebe, Rattenhirn-/-lebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Osteosarkomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

14195-1-AP bindet in WB, IHC, IF, CoIP, ELISA CAT-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAT-1 fusion protein Ag5398
• Vollständiger Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 67-70 kDa
• GenBank-Zugangsnummer: BC063303
• Gene symbol: SLC7A1
• Gene ID (NCBI): 6541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell; In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.; Authors - Hongling Huang ; KO Validated
mouse	IHC	J Cachexia Sarcopenia Muscle; Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.; Authors - Thomas Kunzke
mouse, human	WB	J Steroid Biochem Mol Biol; High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.; Authors - Rui Wang
human	WB	Mol Cell Proteomics; Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.; Authors - James R Byrnes
human	IHC	Front Oncol; An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma.; Authors - Zhuo Lin
rat	IHC	Exp Neurol; Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra.; Authors - Cannon Jason R JR