Serotonin transporter Polyklonaler Antikörper

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• KD/KO Validated

Serotonin transporter Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19559-1-AP

Synonyme

5 HTT, 5 HTTLPR, 5HT transporter, 5HTT, hSERT, HTT, OCD1, serotonin transporter, SERT, SLC6A4

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Galerie der Validierungsdaten

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  WB analysis using 19559-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 19559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 19559-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19559-1-AP (Serotonin transporter antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 19559-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19559-1-AP (Serotonin transporter antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

19559-1-AP bindet in WB, IHC, ELISA Serotonin transporter und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa and 140 kDa
• GenBank-Zugangsnummer: NM_001045
• Gene symbol: SLC6A4
• Gene ID (NCBI): 6532
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 and 140 kDa bands in SDS-PAGE. The 140 kDa band corresponds to the dimer of high mannose-type SERT (PMID: 19323988).

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.; Authors - Jane L Tarry-Adkins
mouse	WB	Sci Rep; Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability.; Authors - Yicong Li ; KO Validated
mouse	WB	J Cell Physiol; Serotonin Transporter Protects the Placental Cells Against Apoptosis in Caspase 3-Independent Pathway.; Authors - Coedy Hadden ; KO Validated
mouse	WB	Psychopharmacology (Berl); Detection of the role of intestinal flora and tryptophan metabolism involved in antidepressant-like actions of crocetin based on a multi-omics approach; Authors - Susu Lin
mouse	WB	Biomed Pharmacother; Prophylactic effect of Tongxieyaofang polysaccharide on depressive behavior in adolescent male mice with chronic unpredictable stress through the microbiome-gut-brain axis; Authors - Helin Chen
human	IHC,WB	Mol Med Rep; Identification of potential prognostic markers for lung adenocarcinoma using comprehensive analysis; Authors - Liang Huang