Band 3/ AE1 Polyklonaler Antikörper

Band 3/ AE1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 28131-1-AP

Synonyme

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, band 3, Band 3 anion transport protein, band 3/ AE1, CD233, SLC4A1

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 28131-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 28131-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28131-1-AP (SLC4A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28131-1-AP (band 3/ AE1antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 28131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (28131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human placenta using 28131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (28131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

28131-1-AP bindet in WB, IP, IHC, IF, ELISA Band 3/ AE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Band 3/ AE1 fusion protein Ag27878
• Vollständiger Name: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Berechnetes Molekulargewicht: 911 aa, 102 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC101570
• Gene symbol: SLC4A1
• Gene ID (NCBI): 6521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

Publikationen

Species	Application	Title
human	WB	Mol Cell; The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.; Authors - Eunus S Ali
human	IP	Int J Hematol; Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.; Authors - Akihito Kitao
mouse	IF	eLife; Piezo1 as a force-through-membrane sensor in red blood cells; Authors - George Vaisey
human	IF	NPJ Regen Med; Ferric citrate and apo-transferrin enable erythroblast maturation with β-globin from hemogenic endothelium; Authors - Soo-Been Jeon