Band 3/ AE1 Polyklonaler Antikörper

Band 3/ AE1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18566-1-AP

Synonyme

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, band 3, Band 3 anion transport protein, band 3/ AE1, SLC4A1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 18566-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18566-1-AP (band 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 18566-1-AP

  IP Result of anti-band 3 (IP:18566-1-AP, 4ug; Detection:18566-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human spleen using 18566-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18566-1-AP (band 3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18566-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18566-1-AP (band 3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18566-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18566-1-AP (band 3 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 18566-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18566-1-AP (band 3 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 18566-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18566-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18566-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18566-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 18566-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (18566-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 18566-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (18566-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Magenkrebsgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18566-1-AP bindet in WB, IP, IHC, IF, ELISA Band 3/ AE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Plasmodium falciparum
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Band 3/ AE1 fusion protein Ag13076
• Vollständiger Name: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Berechnetes Molekulargewicht: 911 aa, 102 kDa
• Beobachtetes Molekulargewicht: 95 kDa, 50 kDa
• GenBank-Zugangsnummer: BC101570
• Gene symbol: SLC4A1
• Gene ID (NCBI): 6521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

Publikationen

Species	Application	Title
mouse	WB	Blood; Ferroportin deficiency in erythroid cells causes serum iron deficiency and promotes hemolysis due to oxidative stress.; Authors - De-Liang Zhang
mouse	WB	Sci Adv; Red blood cell-derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy.; Authors - Xiao Han
mouse,human	WB	J Proteome Res; Proximity Proteomics Has Potential for Extracellular Vesicle Identification.; Authors - Hisako Kaneda
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
rat	IHC	PLoS One; Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.; Authors - Liddelow Shane A SA
rat	IHC	Front Neurosci; Influx mechanisms in the embryonic and adult rat choroid plexus: a transcriptome study.; Authors - Norman R Saunders