SLC40A1/FPN1 Polyklonaler Antikörper

SLC40A1/FPN1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

50

Kat-Nr. : 26601-1-AP

Synonyme

Ferroportin 1, FPN1, HFE4, IREG1, Iron regulated transporter 1, MST079, MSTP079, MTP1, SLC11A3, SLC40A1

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Galerie der Validierungsdaten

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  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26601-1-AP (SLC40A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26601-1-AP (SLC40A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26601-1-AP (SLC40A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse small intestine using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26601-1-AP (SLC40A1/FPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293T-Zellen, HepG2-Zellen, Mauslebergewebe, Mausmilzgewebe, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 48 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

26601-1-AP bindet in WB, IHC, IF, ELISA SLC40A1/FPN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC40A1/FPN1 fusion protein Ag24273
• Vollständiger Name: solute carrier family 40 (iron-regulated transporter), member 1
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 62-70 kDa
• GenBank-Zugangsnummer: BC037733
• Gene symbol: SLC40A1
• Gene ID (NCBI): 30061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC40A1, also named as Ferroportin 1 (FPN1), is an iron transporter which is involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. It has been reported that SLC40A1 mutations may lead to macrophage iron overload, hyperferritinemia, and normal transferrin saturation among ferroportin iron overload patients. This antibody detects 62-70 kDa protein as well as the fragments of 56 kDa and 35 kDa protein similar to papers in SDS-PAGE. (PMID: 21396368,16054062, 18974313)

Publikationen

Species	Application	Title
human	WB	Redox Biol; NCOA4-mediated ferritinophagy is involved in ionizing radiation-induced ferroptosis of intestinal epithelial cells.; Authors - Hao Zhou
human	WB	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
human	WB	Theranostics; Holo-lactoferrin: the link between ferroptosis and radiotherapy in triple-negative breast cancer.; Authors - Zheng Zhang
human	WB	Redox Biol; miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia.; Authors - Heng Zhang
mouse	WB	Cell Chem Biol; 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.; Authors - Chenwen Shao
human	WB	Oxid Med Cell Longev; HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation.; Authors - Shangting Han