ZIP7 Polyklonaler Antikörper

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• KD/KO Validated

ZIP7 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 19429-1-AP

Synonyme

KE4, RING5, SLC39A7, Zinc transporter SLC39A7, ZIP 7, ZIP7

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Galerie der Validierungsdaten

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  WB analysis using 19429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19429-1-AP (ZIP7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19429-1-AP

  IP Result of anti-ZIP7 (IP:19429-1-AP, 4ug; Detection:19429-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19429-1-AP (ZIP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19429-1-AP (ZIP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19429-1-AP (ZIP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19429-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19429-1-AP (ZIP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Produktinformation

19429-1-AP bindet in WB, IP, IHC, IF, ELISA ZIP7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZIP7 fusion protein Ag13762
• Vollständiger Name: solute carrier family 39 (zinc transporter), member 7
• Berechnetes Molekulargewicht: 469 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa, 56 kDa
• GenBank-Zugangsnummer: BC000645
• Gene symbol: SLC39A7
• Gene ID (NCBI): 7922
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)

Publikationen

Species	Application	Title
mouse	WB	Chem Sci; X-ray fluorescence imaging reveals subcellular biometal disturbances in a childhood neurodegenerative disorder.; Authors - A Grubman
human	WB	Cell Death Differ; Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking.; Authors - Astrid Fauster
mouse	WB,IF	Acta Neuropathol Commun; Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.; Authors - Alexandra Grubman ; KD Validated
mouse	IF	J Biol Chem; PMID: 25969539; Authors - Ying Liu
mouse	WB	Metallomics; Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses?; Authors - Alexandra Grubman
human	WB	J Nutr Biochem; B cell activation and proliferation increase intracellular zinc levels.; Authors - Johanna Ollig