LIV-1/ZIP6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LIV-1/ZIP6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14236-1-AP

Synonyme

Ermelin, LIV 1, LIV1, LIV-1/ZIP6, SLC39A6, Zinc transporter ZIP6, ZIP 6, ZIP6, Zrt and Irt like protein 6

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Galerie der Validierungsdaten

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  WB analysis using 14236-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14236-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14236-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14236-1-AP

  WB result of LIV-1/ZIP6 antibody (14236-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIV-1/ZIP6 transfected HeLa cells.

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  IHC staining of human pancreas cancer using 14236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14236-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, A549-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

14236-1-AP bindet in WB, IHC, IF, ELISA LIV-1/ZIP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIV-1/ZIP6 fusion protein Ag5478
• Vollständiger Name: solute carrier family 39 (zinc transporter), member 6
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa, 48 and 32 kDa
• GenBank-Zugangsnummer: BC039498
• Gene symbol: SLC39A6
• Gene ID (NCBI): 25800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The zinc transporter LIV-1 (also known as SLC39A6 or ZIP6) was originally identified as an estrogen-induced gene in breast cancer cells. Elevated LIV-1 expression is reportedly related to cancer progression in various types of cancer, including breast, prostate, pancreatic, cervical and liver cancers. While correlation of LIV-1 and prognosis may vary. LIV 1 has 3 isoforms with MW of 85-100 (glycosylation and phosphorylation), 48 and 32 kDa while migrates as different bands in different lysates in SDS-PAGE.

Publikationen

Species	Application	Title
human	WB,IHC	Nat Genet; Genome-wide association study identifies common variants in SLC39A6 associated with length of survival in esophageal squamous-cell carcinoma.; Authors - Wu Chen C ; KD Validated
human	WB	Gastroenterology; Solute Carrier Family 39 Member 6 Gene Promotes Aggressiveness of Esophageal Carcinoma Cells by Increasing Intracellular Levels of Zinc, Activating Phosphatidylinositol 3-Kinase Signaling, and Up-regulating Genes That Regulate Metastasis.; Authors - Xinxin Cheng ; KD Validated
human	WB	Oncotarget; miR-192, a prognostic indicator, targets the SLC39A6/SNAIL pathway to reduce tumor metastasis in human hepatocellular carcinoma.; Authors - Junwei Lian
human	WB,IF	Oncotarget; Co-expression analysis revealed PTCH1-3'UTR promoted cell migration and invasion by activating miR-101-3p/SLC39A6 axis in non-small cell lung cancer: implicating the novel function of PTCH1.; Authors - Xuechao Wan ; KD Validated
human	WB, IHC	J Transl Med; SLC39A6: a potential target for diagnosis and therapy of esophageal carcinoma.; Authors - Xiao-Bin Cui
human	WB,IHC	Oncol Lett; Role of SLC39A6 in the development and progression of liver cancer.; Authors - Zhen Wan ; KD Validated