SLC35A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC35A1 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16342-1-AP

Synonyme

CMP SA Tr, CMP Sia Tr, CMP sialic acid transporter, CMPST, CST, hCST, inactive, SLC35A1

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Galerie der Validierungsdaten

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  IHC staining of mouse spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16342-1-AP (SLC35A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16342-1-AP (SLC35A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16342-1-AP (SLC35A1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16342-1-AP (SLC35A1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16342-1-AP (SLC35A1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SLC35A1 antibody (16342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Hautgewebe, humanes Milzgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16342-1-AP bindet in WB, IHC, IF, ELISA SLC35A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC35A1 fusion protein Ag9524
• Vollständiger Name: solute carrier family 35 (CMP-sialic acid transporter), member A1
• Berechnetes Molekulargewicht: 337 aa, 37 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC017807
• Gene symbol: SLC35A1
• Gene ID (NCBI): 10559
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Solute carrier family 35 member A1 (SLC35A1) , also named as CMP-sialic acid transporter (CST) , is responsible for transferring CMP-sialic acid from the cytosol through Golgi membranes, thus playing a vital role in the terminal sialylation of glycan structures. Slc35a1 deficiency causes thrombocytopenia due to impaired megakaryocytopoiesis and excessive platelet clearance in the liver. In addition, SCL35A1 acts independently from sialic acid in functional α-DG O-mannosylation(PMID: 25552652).

Publikationen

Species	Application	Title
human	IHC	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
human	WB, IF	Cells; Knockout of the CMP-Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays.; Authors - Antje Banning ; KO Validated
human	WB	Cell Rep; A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection.; Authors - Jessie Kulsuptrakul ; KO Validated