SLC2A9 Monoklonaler Antikörper

SLC2A9 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2D6E1

Kat-Nr. : 67530-1-Ig

Synonyme

Glucose transporter type 9, GLUT 9, GLUT9, GLUTX, SLC2A9, UAQTL2, URATv1

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Galerie der Validierungsdaten

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  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human hepatocirrhosis using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human hepatocirrhosis tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human hepatocirrhosis using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human hepatocirrhosis tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SMMC-7721-Zellen, Hausschwein-Lebergewebe, HepG2-Zellen, HuH-7-Zellen, Kaninchenlebergewebe, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberzirrhosegewebe, humanes Nierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

67530-1-Ig bindet in WB, IHC, IF, ELISA SLC2A9 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLC2A9 fusion protein Ag24135
• Vollständiger Name: solute carrier family 2 (facilitated glucose transporter), member 9
• Berechnetes Molekulargewicht: 540 aa, 59 kDa
• Beobachtetes Molekulargewicht: 56-59 kDa
• GenBank-Zugangsnummer: BC018897
• Gene symbol: SLC2A9
• Gene ID (NCBI): 56606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IHC	Evid Based Complement Alternat Med; Effects of a Macroporous Resin Extract of Dendrobium officinale Leaves in Rats with Hyperuricemia Induced by Anthropomorphic Unhealthy Lifestyle; Authors - Lin-Zi Li
mouse	WB	J Pharm Pharmacol; Antihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice.; Authors - Wen-Hao Xu
mouse	WB	Curr Res Food Sci; Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease; Authors - Yan Huang
human,mouse	WB	Biomed Pharmacother; Sanghuangporus vaninii ethanol extract alleviates hyperuricemic renal injury by regulating the uric acid transporters and inhibiting HK-2 apoptosis; Authors - Liping Hua