SLC19A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC19A1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 25958-1-AP

Synonyme

CHMD, FLOT1, Folate transporter 1, FOLT, IFC 1, IFC1, Intestinal folate carrier 1, Placental folate transporter, Reduced folate carrier protein, REFC, RFC, RFC1, SLC19A1

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Galerie der Validierungsdaten

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  WB analysis using 25958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25958-1-AP (SLC19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25958-1-AP (SLC19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

25958-1-AP bindet in WB, IHC, ELISA SLC19A1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC19A1 fusion protein Ag22921
• Vollständiger Name: solute carrier family 19 (folate transporter), member 1
• Berechnetes Molekulargewicht: 591 aa, 65 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC003068
• Gene symbol: SLC19A1
• Gene ID (NCBI): 6573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC19A1, also named as RFC (reduced folate carrier), is an anionic exchanger that involved in the regulation of intracellular concentrations of folate in exchange for anions. The predicted MW of  SLC19A1 is 65 kDa and 25958-1-AP antibody recognizes the 46 kDa protein which is similar to papers. The 38-40 kDa minor forms and 58 kDa form have also been reported that could be alternate splicing, post-transcriptional modification or the role of accessory proteins. (PMID: 17404734, 9111015)

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP; Authors - Xiaobo Li ; KD Validated
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

It does not show a clear band around 46kDa for SLC19A1. It does not work in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: HepG2, mouse liver and brain

SLC19A1 Antibody Western Blot validation (1:400 dilution) in HepG2, mouse liver and brain (Cat no:25958-1-AP)