MCT2 Polyklonaler Antikörper

MCT2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20355-1-AP

Synonyme

MCT2, SLC16A7

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 20355-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20355-1-AP (MCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20355-1-AP (MCT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 20355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20355-1-AP (MCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20355-1-AP (MCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

20355-1-AP bindet in WB, IHC, IF, ELISA MCT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCT2 fusion protein Ag14201
• Vollständiger Name: solute carrier family 16, member 7 (monocarboxylic acid transporter 2)
• Berechnetes Molekulargewicht: 478 aa, 52 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 40-52 kDa
• GenBank-Zugangsnummer: BC030693
• Gene symbol: SLC16A7
• Gene ID (NCBI): 9194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC16A7, also named as MCT2, is a proton-coupled monocarboxylate transporter catalyzing the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A7 is the primary monocarboxylate transporter in Purkinje cells and can be detected in brain, cerebellum, liver and kidney, but not in vascular smooth muscle cells, pericytes, endothelium, heart, lung or stomach. There are some isoforms of SLC16A7 with bands around 52 kDa and 40-45 kDa in SDS-PAGE. And the 80 kDa protein might be the dimer which is similar to the papers published. (PMID: 9482213, 15749979, 11291733, 16901736)

Publikationen

Species	Application	Title
human	WB	Br J Cancer; Autoregulation of H+/lactate efflux prevents monocarboxylate transport (MCT) inhibitors from reducing glycolytic lactic acid production.; Authors - Wiktoria Blaszczak
rat	WB, IF	Anim Cells Syst (Seoul); MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke.; Authors - Xiaorong Yu
human	WB	J Exp Clin Cancer Res; ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.; Authors - Shuohui Dong
mouse	WB	Biomed Pharmacother; Fibroblast growth factor 21 protects the liver from apoptosis in a type 1 diabetes mouse model via regulating L-lactate homeostasis; Authors - Jiapin Yan