SLC16A4 Polyklonaler Antikörper

SLC16A4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20889-1-AP

Synonyme

MCT 4, MCT 5, MCT4, MCT5, Monocarboxylate transporter 4, Monocarboxylate transporter 5, SLC16A4

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 20889-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20889-1-AP (SLC16A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20889-1-AP (SLC16A4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 20889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 20889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 20889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 20889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20889-1-AP (SLC16A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20889-1-AP bindet in WB, IHC, ELISA SLC16A4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC16A4 fusion protein Ag14190
• Vollständiger Name: solute carrier family 16, member 4 (monocarboxylic acid transporter 5)
• Berechnetes Molekulargewicht: 487 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC021664
• Gene symbol: SLC16A4
• Gene ID (NCBI): 9122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC16A4, also named as MCT5, is a proton-linked monocarboxylate transporter which can catalyze the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A4 is expressed in human intestine, liver and kidney. (PMID:18996437)

Publikationen

Species	Application	Title
mouse	WB	Arq Bras Cardiol; SLC26A4-AS1 Aggravates AngII-induced Cardiac Hypertrophy by Enhancing SLC26A4 Expression; Authors - Xiaoliang Han
human	WB	Int J Biochem Cell Biol; The CDK4/6 inhibitor PD0332991 stabilizes FBP1 by repressing MAGED1 expression in pancreatic ductal adenocarcinoma.; Authors - Bin Zhang