SLC16A12 Polyklonaler Antikörper

SLC16A12 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20553-1-AP

Synonyme

CJMG, MCT 12, MCT12, Monocarboxylate transporter 12, SLC16A12

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 20553-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20553-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Hodengewebe, humanes Nierengewebe, Maushodengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

20553-1-AP bindet in WB, IHC, ELISA SLC16A12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 16, member 12 (monocarboxylic acid transporter 12)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53-56 kDa
• GenBank-Zugangsnummer: NM_213606
• Gene symbol: SLC16A12
• Gene ID (NCBI): 387700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC16A12, also named as MCT12 and CJMG, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. It is a proton-linked monocarboxylate transporter. SLC16A12 catalyzes the rapid transport across the plasma membrane of many monocarboxylates. The antibody is specific to SLC16A12.

Publikationen

Species	Application	Title
Xenopus	WB	Biochem Biophys Res Commun; Molecular characterization of the orphan transporter SLC16A9, an extracellular pH- and Na+-sensitive creatine transporter.; Authors - Yuya Futagi
human	IHC	Medicine (Baltimore); Decreased expression of SLC16A12 mRNA predicts poor prognosis of patients with clear cell renal cell carcinoma.; Authors - Jie Mei