KCC2/SLC12A5-Specific Polyklonaler Antikörper

KCC2/SLC12A5-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 19565-1-AP

Synonyme

hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5-Specific, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

---

Galerie der Validierungsdaten

• 

  WB analysis of human brain using 19565-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 19565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody (19565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 19565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody (19565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

19565-1-AP bindet in WB, IHC, IF, ELISA KCC2/SLC12A5-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 12 (potassium-chloride transporter), member 5
• Berechnetes Molekulargewicht: 126 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: NM_020708
• Gene symbol: SLC12A5
• Gene ID (NCBI): 57468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)

Publikationen

Species	Application	Title
human,mouse	WB,IF	Neurobiol Dis; HIV and opiates dysregulate K+- Cl- cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice.; Authors - Aaron J Barbour
mouse	IF	iScience; Time-restricted feeding entrains long-term behavioral changes through the IGF2-KCC2 pathway.; Authors - Qiaocheng Zhai
mouse	WB，IF	ASN Neuro; Restoration of KCC2 Membrane Localization in Striatal Dopamine D2 Receptor-Expressing Medium Spiny Neurons Rescues Locomotor Deficits in HIV Tat-Transgenic Mice.; Authors - Aaron J Barbour
rat	WB	Brain Behav; Involvement of the BDNF-TrkB-KCC2 pathway in neuropathic pain after brachial plexus avulsion.; Authors - Shuo Zhao
mouse	IHC	Front Mol Neurosci; Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion; Authors - Haijun Hu