DMT1 Polyklonaler Antikörper

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• KD/KO Validated

DMT1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

71

Kat-Nr. : 20507-1-AP

Synonyme

DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT 1, DMT1, NRAMP 2, NRAMP2, SLC11A2

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Galerie der Validierungsdaten

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  WB analysis using 20507-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 20507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20507-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20507-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 20507-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20507-1-AP (DMT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 20507-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20507-1-AP (SLC11A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 20507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20507-1-AP (SLC11A2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse small intestine using 20507-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using DMT1 antibody (20507-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 20507-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DMT1 antibody (20507-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, Caco-2-Zellen, COLO 320-Zellen, Neuro-2a-Zellen, Rattennierengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Dünndarmgewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 66 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

20507-1-AP bindet in WB, IHC, IF, ELISA DMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DMT1 fusion protein Ag14314
• Vollständiger Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• Berechnetes Molekulargewicht: 568 aa, 62 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC002592
• Gene symbol: SLC11A2
• Gene ID (NCBI): 4891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that play a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including the brain, kidney, testis, duodenum, and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Publikationen

Species	Application	Title
human	WB	Blood; Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.; Authors - Floriane Petit
human	IHC	Acta Neuropathol; Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.; Authors - Till S Zimmer
mouse	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
mouse	IF	Nat Commun; Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.; Authors - Hirofumi Yamaguchi
rat	WB	Transl Res; Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.; Authors - Guang Yang
mouse	WB	Redox Biol; Apolipoprotein E deficiency induces a progressive increase in tissue iron contents with age in mice.; Authors - Juan Ma