SKIL Polyklonaler Antikörper

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• KD/KO Validated

SKIL Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 19218-1-AP

Synonyme

SKI like oncogene, Ski like protein, Ski related oncogene, Ski related protein, SKIL, SNO, SnoA, SnoI, SnoN

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Galerie der Validierungsdaten

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  WB analysis using 19218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 19218-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19218-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 19218-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19218-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 19218-1-AP

  IP Result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IP experiment of HepG2 using 19218-1-AP

  IP Result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human endometrial cancer using 19218-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 19218-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 19218-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19218-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 19218-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HepG2-Zellen, humanes Skelettmuskelgewebe, MCF-7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

19218-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA SKIL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SKIL fusion protein Ag5282
• Vollständiger Name: SKI-like oncogene
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC059386
• Gene symbol: SKIL
• Gene ID (NCBI): 6498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.

Publikationen

Species	Application	Title
human	CoIP	Cell Death Differ; PIAS1 and TIF1γ collaborate to promote SnoN SUMOylation and suppression of epithelial-mesenchymal transition.; Authors - Ayan Chanda
mouse	WB	J Med Chem; Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.; Authors - Jin Zhang
		J Neurosci; The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain.; Authors - Xiaoying Chen
human	WB	Mol Cell Proteomics; Quantitative ubiquitylome analysis reveals specificity of RNF111/Arkadia E3 ubiquitin ligase for its degradative substrates SKI and SKIL/SnoN in TGF-β signaling pathway; Authors - Victor Laigle
human	WB,IHC,IF	Apoptosis; SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.; Authors - Zuo-Long Wu ; KD Validated
human	WB	J Cell Mol Med; MDM2 is implicated in high-glucose-induced podocyte mitotic catastrophe via Notch1 signalling.; Authors - Hui Tang