SIRT7 Polyklonaler Antikörper

SIRT7 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 29729-1-AP

Synonyme

SIR2 like protein 7, SIR2L7, SIRT7

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Galerie der Validierungsdaten

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  WB analysis using 29729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 29729-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIRT7 antibody (29729-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HCT 116-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

29729-1-AP bindet in WB, IHC, IF, ELISA SIRT7 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRT7 fusion protein Ag31794
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Berechnetes Molekulargewicht: 45KD
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_016538
• Gene symbol: SIRT7
• Gene ID (NCBI): 51547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Publikationen

Species	Application	Title
human,rat	WB,IHC	Int Urol Nephrol; SIRT7 silencing by miR-152-3p confers cell apoptosis and renal functional impairment induced by renal ischaemia/reperfusion injury; Authors - Yan Wang