SIRP Alpha Polyklonaler Antikörper

SIRP Alpha Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14482-1-AP

Synonyme

BIT, CD172A, Inhibitory receptor SHPS 1, Macrophage fusion receptor, MFR, MYD 1, MyD 1 antigen, MYD1, P84, PTPNS1, SHP substrate 1, SHPS 1, SHPS1, SIRP, SIRP alpha, SIRP ALPHA 1, Sirp alpha 2, Sirp alpha 3, SIRPA, SIRPalpha, SIRPalpha2

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Galerie der Validierungsdaten

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  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 14482-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Maus-Cerebellum-Gewebe, PC-12-Zellen, THP-1-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

14482-1-AP bindet in WB, ELISA SIRP Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRP Alpha fusion protein Ag5865
• Vollständiger Name: signal-regulatory protein alpha
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC033092
• Gene symbol: SIRPA
• Gene ID (NCBI): 140885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

Publikationen

Species	Application	Title
mouse	WB	Cell Prolif; Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling; Authors - Qing Tu
mouse	WB	Acta Biochim Biophys Sin (Shanghai); Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6.; Authors - Kejia Zhao
human	WB	J Obstet Gynaecol Res; Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages.; Authors - Qi Xie
human	WB	Biomedicines; Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis; Authors - Ying Jiang