SHH Polyklonaler Antikörper

SHH Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29752-1-AP

Synonyme

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

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Galerie der Validierungsdaten

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  WB analysis using 29752-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29752-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse embryo using 29752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 29752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 29752-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHH (29752-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, MCF-7-Zellen, MDA-MB-231-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29752-1-AP bindet in WB, IHC, FC, ELISA SHH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHH fusion protein Ag31338
• Vollständiger Name: sonic hedgehog homolog (Drosophila)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: NM_000193
• Gene symbol: SHH
• Gene ID (NCBI): 6469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SHH, Sonic hedgehog protein, binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PMID: 10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PMID: 10753901). SHH is synthesized as a 45 kDa precursor that undergoes an autocatalytic processing event that produces a 19 kDa N-terminal product, responsible for all signaling activities, and a 25 kDa C-terminal fragment (PMID: 10753901, PMID: 16282375).