SHD Polyklonaler Antikörper

SHD Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20814-1-AP

Synonyme

SHD

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 20814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 20814-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20814-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 20814-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Neuro-2a using 20814-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Neuro-2a cells using 20814-1-AP (SHD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Neuro-2a-Zellen, NIH/3T3-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IF: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20814-1-AP bindet in WB, IF,ELISA SHD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHD fusion protein Ag14794
• Vollständiger Name: Src homology 2 domain containing transforming protein D
• Berechnetes Molekulargewicht: 340 aa, 38 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC007206
• Gene symbol: SHD
• Gene ID (NCBI): 56961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.