SH3GLB2 Monoklonaler Antikörper

SH3GLB2 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

4B1A10

Kat-Nr. : 68325-1-Ig

Synonyme

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

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Galerie der Validierungsdaten

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  WB analysis using 68325-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68325-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IP experiment of HeLa using 68325-1-Ig

  IP result of anti-SH3GLB2(IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug.

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  IHC staining of mouse testis using 68325-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 68325-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68325-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68325-1-Ig bindet in WB, IP, IHC, IF, ELISA SH3GLB2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SH3GLB2 fusion protein Ag8932
• Vollständiger Name: SH3-domain GRB2-like endophilin B2
• Berechnetes Molekulargewicht: 395 aa, 44 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC014635
• Gene symbol: SH3GLB2
• Gene ID (NCBI): 56904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).