SH3BP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SH3BP1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20541-1-AP

Synonyme

3BP 1, SH3 domain binding protein 1, SH3BP1

---

Galerie der Validierungsdaten

• 

  WB analysis using 20541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 20541-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 20541-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20541-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20541-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20541-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20541-1-AP

  Immunofluorescent analysis of HepG2 cells, using SH3BP1 antibody 20541-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 20541-1-AP

  Immunofluorescent analysis of Hela cells, using SH3BP1 antibody 20541-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Mauslungengewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

20541-1-AP bindet in WB, IF, ELISA SH3BP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH3BP1 fusion protein Ag14575
• Vollständiger Name: SH3-domain binding protein 1
• Berechnetes Molekulargewicht: 701 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76-80 kDa, 51 kDa
• GenBank-Zugangsnummer: BC008282
• Gene symbol: SH3BP1
• Gene ID (NCBI): 23616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.; Authors - Daniel Schlam
human	WB	Cancer Med; Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1 regulate drug resistance in chronic myeloid leukemia.; Authors - Kibeom Park ; KD Validated