SH3BGRL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SH3BGRL2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21944-1-AP

Synonyme

FASH3, SH3BGRL2

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 21944-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21944-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21944-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21944-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 21944-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Plazenta-Gewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Plazenta-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

21944-1-AP bindet in WB, IHC, IF, ELISA SH3BGRL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH3BGRL2 fusion protein Ag16638
• Vollständiger Name: SH3 domain binding glutamic acid-rich protein like 2
• Berechnetes Molekulargewicht: 107 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC040489
• Gene symbol: SH3BGRL2
• Gene ID (NCBI): 83699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Am J Cancer Res; SH3BGRL2 exerts a dual function in breast cancer growth and metastasis and is regulated by TGF-β1.; Authors - Dou-Dou Li ; KD Validated
human	WB	Front Pharmacol; Astragalus Polysaccharides Inhibits Tumorigenesis and Lipid Metabolism Through miR-138-5p/SIRT1/SREBP1 Pathway in Prostate Cancer.; Authors - Shanqi Guo