SGTA Monoklonaler Antikörper

SGTA Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10B11

Kat-Nr. : 60305-1-Ig

Synonyme

Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

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Galerie der Validierungsdaten

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  WB analysis using 60305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of NIH/3T3 using 60305-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 60305-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 60305-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 60305-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 60305-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 60305-1-Ig

  Immunofluorescent analysis of HeLa cells using 60305-1-Ig (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60305-1-Ig bindet in WB, IHC, IF, ELISA SGTA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SGTA fusion protein Ag1475
• Vollständiger Name: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34-37 kDa
• GenBank-Zugangsnummer: BC008885
• Gene symbol: SGTA
• Gene ID (NCBI): 6449
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.