SGK1 Polyklonaler Antikörper

SGK1 Polyklonal Antikörper für FC, IF, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 23394-1-AP

Synonyme

SGK, SGK1

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Galerie der Validierungsdaten

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  IP experiment of HEK-293 using 23394-1-AP

  IP Result of anti-SGK1 (IP:23394-1-AP, 4ug; Detection:23394-1-AP 1:600) with HEK-293 cells lysate 1480ug.

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  IHC staining of human pancreas using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

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  IF Staining of HEK-293 using 23394-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23394-1-AP (SGK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 23394-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SGK1 antibody (23394-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

23394-1-AP bindet in IP, IHC, IF, FC, ELISA SGK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGK1 fusion protein Ag17888
• Vollständiger Name: serum/glucocorticoid regulated kinase 1
• Berechnetes Molekulargewicht: 431 aa, 49 kDa
• GenBank-Zugangsnummer: BC001263
• Gene symbol: SGK1
• Gene ID (NCBI): 6446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum- and glucocorticoid-induced kinase 1 (SGK1),also named as SGK, is a S/T protein kinase that belongs to the AGC (cAMP-dependent, cGMP-dependent, and protein kinase C) kinase family. It is expressed in many cell types and participates in numerous cellular processes and it is ubiquitinated and degraded at the ER membrane(PMID: 16847254). The N-terminal motif of SGK1 is critical for its ubiquitination and degradation(PMID:16817852 ). SGK and Akt are likely to phosphorylate related substrates, as they share a similar consensus phosphorylation site (RXRXXS/T)(PMID:11154281). It has 5 isoforms produced by alternative promoter usage and alternative splicing.

Publikationen

Species	Application	Title
human		FASEB J; Inhibition of serum- and glucocorticoid-inducible kinase 1 enhances TLR-mediated inflammation and promotes endotoxin-driven organ failure.; Authors - Huaxin Zhou
mouse		J Innate Immun; Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.; Authors - Yunzhi Liu
mouse		J Immunol; Serum- and Glucocorticoid-Inducible Kinase 1 Promotes Alternative Macrophage Polarization and Restrains Inflammation through FoxO1 and STAT3 Signaling.; Authors - Junling Ren
mouse	IHC	J Transl Med; Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3; Authors - Xiangdong Li
human		Appl Biochem Biotechnol; Long Non-coding RNA ZFAS1 Regulates Fibrosis and Scortosis in the Cell Model of Diabetic Nephropathy Through miR-525-5p/SGK1 Axis; Authors - Langen Zhuang