SFRP1 Polyklonaler Antikörper

SFRP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 26460-1-AP

Synonyme

FRP, FRP 1, FRP1, FrzA, SARP 2, SARP2, sFRP 1, SFRP1

---

Galerie der Validierungsdaten

• 

  WB analysis using 26460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26460-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 26460-1-AP (SFRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maushodengewebe, Mausnierengewebe, NIH/3T3-Zellen, Rattennierengewebe, SH-SY5Y-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

26460-1-AP bindet in WB, IHC, IF, ELISA SFRP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFRP1 fusion protein Ag24844
• Vollständiger Name: secreted frizzled-related protein 1
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC036503
• Gene symbol: SFRP1
• Gene ID (NCBI): 6422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.; Authors - Liansheng Sha
rat	IHC	Cells; Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells.; Authors - Keita Ipposhi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hua (Verified Customer) (03-14-2023)

There are doubts about if this antibody is working in WB. There are some non-specific bands and the supposed band is very weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain

SFRP1 Antibody Western Blot validation (1:2000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:26460-1-AP)